Potassium conductance changes in skeletal muscle and the potassium concentration in the transverse tubules
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Ouabain binding and coupled sodium, potassium, and chloride transport in isolated transverse tubules of skeletal muscle
The affinity and number of binding sites of [3H]ouabain to isolated transverse (T) tubules were determined in the absence and presence of deoxycholate. In both conditions the KD was approximately 53 nM while deoxycholate increased the number of binding sites from 3.5 to 37 pmol/mg protein. We concluded that the ouabain binding sites were located primarily on the inside of the isolated vesicle a...
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A dissection of the semitendinosus muscle from Rana pipiens was developed for three-microelectrode voltage-clamp studies of the delayed potassium-selective conductance system. The delayed conductance inactivates in muscles bathed in high concentrations of potassium or rubidium, but can be reprimed by hyperpolarizing voltage pulses to membrane potentials beyond -80 mV. The repriming time-course ...
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A two-microelectrode voltage clamp and optical measurements of membrane potential changes at the transverse tubular system (TTS) were used to characterize delayed rectifier K currents (IK(V)) in murine muscle fibers stained with the potentiometric dye di-8-ANEPPS. In intact fibers, IK(V) displays the canonical hallmarks of K(V) channels: voltage-dependent delayed activation and decay in time. T...
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چکیده ندارد.
15 صفحه اولSkeletal muscle magnesium and potassium in asthmatics
Skeletal muscle magnesium and potassium in asthmatics treated with oral beta2-agonists. T. Gustafson, K. Boman, L. Rosenhall, T. Sandström, P.O. Wester. ERS Journals Ltd 1996. ABSTRACT: Dietary magnesium has been shown to be important for lung function and bronchial reactivity. Interest in electrolytes in asthma has so far mainly been focused upon serum potassium, especially linked to β2-agoni...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1972
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1972.sp009928